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Structural aspects of 3,3'-diiodo-L-thyronine binding to human serum transthyretin
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The effect of high pressure on the membrane association of different types of peripherally bound and integral membrane proteins have been reported, and their pressure behavior is variable (e
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0%AcknowledgmentsMany individuals contributed to the completion of this study
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. An additional experiment in which the incubation time of the protein in the presence of 3 M GdnHCl was increased from 90 min to 24 h showed that the small dimer fraction observed in Fig
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. Due to a rather slow structural unfolding but a fast irreversible step we observe an unfolding process which is under kinetic control
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