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The fluorescence emission of native GR is quite blue-shifted ([lambda]^sub max^ at 317 nm), indicating that, on average, the four tryptophan residues in each GR subunit are well-protected from exposure to the aqueous medium in the folded protein
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8 b shows the distribution of the iwo types of interactions
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Our ability to simulate mimics of denatured-state ensembles will allow us to ask precise questions about the role of the denatured state in the types of applications outlined above
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(34)[FIGURE 3 OMITTED]Efferocytosis also increases the production of secretory leukoprotease inhibitor, which is an antiprotease with activity against elastase and cathepsin G
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As already mentioned above, there is indication from previous studies on several α-amylases that a structural unfolding is followed by an irreversible process (14,22-25)
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4 and 5, it can be seen that the Sn-2
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Our laboratory has previously characterized the affinity between PLCβs and their G-protein activators on model membrane surfaces using FRET (Runnels and Scarlata, 1999)
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193 K/ min (80-11 K/h) as described in Gursky et al
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After washing with PBS (100 [micro]L), biotinylated horse anti-mouse IgG (100 [micro]L) was added to the wells and incubated at room temperature for 20 minutes
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/type (mm)diameter (mm)total egg no
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The 4WJ ribozyme docks with the highest efficiency, i
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