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Control experiments revealed a similar increase in thiol reactivity in the presence of 1 M KCl (data not shown)
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For each amino acid sequence, the form factor was calculated for each snapshot generated from the Monte Carlo simulations
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Remarkably, there is a 20% chance of linding a cavity of radius a = 12
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The results of this study suggest that the ANS binding method may find practical utility in testing the effectiveness of various additives employed in protein formulations as well as to devise safer freeze-drying protocols of pharmaceutical proteins
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Such prefibrillar oligomers could also serve as a common intermediate between the amyloidoses and other misfolding diseases, including prion diseases, Parkinson's disease, and amytrophic lateral sclerosis
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These similar volume changes may correspond to a significant portion of the interaction volume between the two proteins
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This function accounts, in part, for the anti-atherogenic action of HDL and its major protein, apolipoprotein A-1 (apoA-1, 243 aa) (Barter and Rye, 1996)
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Arrhenius plots in(τ) versus 1/T for the two kinetic phases where obtained from the relaxation times τ(T) measured at 70-95°C
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After 48-h incubation, disk reconstitution is incomplete and a significant amount of lipid-poor apoA-2 is detected (lanes 9 and 10)
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The majority of mature amyloids are unbranched fibrils (Rochet and Lansbury, 2000) consisting of several, often intertwined protofilaments (which may form an internal waterfilled canal-Perutz et al
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Similar structures of search ordering would arise in other systems
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The configurational entropy analysis in terms of combined trajectories presented here shall potentially provide accurate estimations of thermodynamic properties of biomolecules given sufficient sampling of conformational space
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He adds that the yellow-green regions of CG4 reveal red-absorbing dust particles that lie between the cloud and Earth
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Denaturing urea gel electrophoresis followed by quantification of RNA bands using phosphorimager analysis and determination of the ratio of cleaved to uncleaved substrate strand as a function of time provided ribozyme activity information
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Because of the similar donor-acceptor distances in the quasi-docked and specifically-docked conformations, it is difficult to distinguish these two species simply on the basis of the overall FRET efficiency
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Each monograph contains six parts: the generic name, pharmacologic class, summary of the fetal risk literature and recommendation, summary of the breast feeding risk literature and recommendation, and available references
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