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Although the thermal unfolding of many proteins can successfully be illustrated by a two-state model, step-wise transitions have been observed in several proteins regardless of whether the process was reversible or irreversible (30,38, 39,49-54)
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The crystal structures of Ca^sup 2+^/CaM show an extended dumbbell shaped molecule, in which its two globular domains are connected with a long α-helix (1)
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van Gunsteren,[dagger] and Alexandre M
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, unequal elevations) which necessitated that the data be fitted with a parallel-lines multiple linear regression model (Fig
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Southern Medical Journal 91, 1038-1041
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