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Notwithstanding the relevance of the phenomenon, little is known of the structure of proteins in ice owing primarily to the inapplicability or the poor sensitivity of usual spectroscopic techniques in this highly scattering and anisotropic medium barber chair .
The newly frozen samples were then allowed to anneal for 12 h at -5°C, followed by a final equilibration of 2 h at the temperature selected for fluorescence measurements (-5 and -13°C) barber chair .
Dynamics of ANS binding to tuna apomyoglobin measured with fluorescence correlation spectroscopy barber chair .
, 2003a), is characteristic of thermodynamically irreversible reactions with high activation energy (Sanchez-Ruiz et al barber chair .
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1 C, curves 2-4; Fig barber chair .
The example of a three-helix bundle is treated in more detail as an illustration barber chair .
The "diffusion-collision" approach, on the other hand, is supported by strong experimental evidence that folding rates are controlled by the rate of diffusion of pieces of the open coil in their search for favorable contacts, rather than a driven collapse along some continuous energy surface (Jacob et al barber chair .
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