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5 C to avoid the possible artifacts caused by large band shifts accompanied with slight intensity changes in this temperature range (see also Fig
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Hence, not many orientations of the glycan chain upon attachment to the protein are possible and we assume that upon energy minimization, the structure obtained mimics the real situation
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FTIR The protein was speed-vacuum dried (Speed-vac rotatory evaporator, Savant Instruments, Farmingdale, NY) and dissolved in the deuterated buffer containing 10OmM NaCI
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The results clearly indicate that the extent of azurin denaturation-aggregation is correlated to the value of n increasing from WT to mutant proteins and that among the latter species it is significantly larger with the cavity-forming mutants I7S and F110S relative to compact C3A/C26A
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Relative to azurin (WT), which is a small tightly packed polypeptide, the intensity enhancement is generally larger (Fig
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One of these, the interaction of CaM with basicHelix-Loop-Helix (bHLH) transcription factors has a 2:2 stoichiometry (11)
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, 2003a) and apoA-1 (Fang et al
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Slowing down the scan rate also leads to a reduction in the CD amplitude in the cooling curves below 80°C (Fig
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A few melanophores were scattered at the base of the pectoral fin bud
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Two opercular spines were also present in all specimens studied
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[Available from INIDEP: P
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The donor-acceptor solutions used were 100 pM in donor concentration, ensuring that virtually all of the detected signals were due to single molecules
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Yaeger PC, Masi TL, Buck de Ortiz JL, et al
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