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INTRODUCTION Aminoacylase, a family of metalloenzymes found in many mammalian tissues and in microorganisms, catalyzes the stereospecific hydrolysis of acylamino acids to yield the corresponding organic acid and amino acid (1-3)
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In contrast, the recombinant protein unfolds via an intermediate
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The excitation and emission wavelengths were fixed at 280 nm and 370 nm
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In fact, the pattern of (τ^sup o^^sub m^)^sub app^ back calculated from the CaM:SMLCK complex when the diffusion tensor would be oriented along an axis perpendicular to the real diffusion tensor closely matches the pattern of (τ^sup o^^sub m^)^sub app^ found for the dimeric CaM:SEF2-1mp complex, Fig
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This ~90° rotation of the diffusion tensor together with the relatively small structural changes within each CaM domain puts direct restraints on the overall structure of the CaM:SEF2-1mp complex, which allows us to choose one of the two possible schematic structure models proposed in our earlier study (11) (see Figs
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