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. Investigation of the properties of these partially folded states using probes such as ANS or bis-ANS (which bind to exposed hydrophobic domains in proteins) was not possible due to the significant overlap between the emission spectra of these probes when bound to proteins and the excitation spectrum of the prosthetic group, FAD (data not shown)
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. Each of the subunits is glycosylated at the Asn-75 position (Fig
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. The supernatant was again subjected to 65% ammonium sulfate fractionation
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. These intrinsic features of RNA folding reactions can be obscured due to the averaging inherent in conventional ensemble experiments
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. Some factors in the interpretation of protein denaturation
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. Statistical mechanics of semi-Hexible chains: A mean held variational approach
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. Studies on the molecular weight and association of Enzyme I
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. Because the spectrum reflects the hydrophobicity of the binding site, shifting to the blue in less polar environments (30,31), the above correlation suggests that extensive binding of ANS is associated with the exposure of hydrophobic regions of the macromolecule that are generally buried and inaccessible to the probe
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. Pressure and propylene carbonate denaturation of native and "aged" phosphorylated cholinesterase
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. ^sup 15^N chemical shifts were indirectly referenced using the gyromagnetic ratio of ^sup 15^N/^sup 1^H (18)
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. The interaction is clearly different from the well-known categories of specific binding by lock-and-key or induced-fit binding, in which the complex in the bound state becomes essentially locked in one conformation
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. Backbone dynamics and energetics of a calmodulin domain mutant exchanging between closed and open conformations
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.1, close to the isoelectric point, the osmotic pressure was lower at the lower NaCl concentrations (0 mM, 100 mM) and was fitted by an adhesive hard sphere model
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. Comparison of the liquid-liquid and literature solid-liquid coexistence curves showed these solutions to be supersaturated and the phase separation to be nonequilibrium in nature
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. The remaining charge of DNA is neutralized by other positively charged chemicals in cell fluid, where mono- and divalent cations comprise up to 1% of the cell weight
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. 1, A''-C'', show the distributions of the T4 DNA long-axis length in these three characteristic regions
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.When pressure studies are carried out in the presence of Gα^sub s^(GDP) subunits are under conditions where the protein will be completely bound (i
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. Mature human plasma HDL and LDL are also stabilized by kinetic barriers, suggesting that such barriers provide a universal natural strategy for lipoprotein stabilization (Mehta et al
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. Careful double mutant studies would be required to identify such nonnative contributions unequivocally
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.02]);* The body was rather elongate instead of moderate (BD ranged from 0
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. There was, however, strong evidence (2-tailed t-test, P<0
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. Supernatant solutions of the cultures were used for the immunostaining assay to select hybridomas producing antibodies reactive to P
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. Because of the similar donor-acceptor distances in the quasi-docked and specifically-docked conformations, it is difficult to distinguish these two species simply on the basis of the overall FRET efficiency
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