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. The data of Kohn et al
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. On freezing of the solution, however, the protein sharply enhances the probe fluorescence and shifts its spectrum to the blue
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. The viscous behavior of globular protein solutions will therefore be strongly influenced by the characteristics of this hydration layer
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. In addition to the divergence of viscosity (Fig
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. The approach to generate these species in solution opens up the possibility to probe the process by a wide number of biochemical and biophysical techniques to provide insight into the mechanism of protein misfolding and misassembly
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