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The free energy of unfolding calculated for rSBA and gSBA as per LEM model are 41 book make permanent up .
In EI^sup sc^, a single peak was observed in CD and DSC experiments, but in the pH range where the thermal transitions could be followed (pH 7-9), two different peaks were not observed; further, the T^sub m^ of the sole transition observed was pH-indcpendent between pH 7 and 9 (Fig book make permanent up .
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In soman-aged HuBChE, the organophosphoryl moiety remains covalently attached to the unfolded protein, and the salt-bridge between the protonated imidazole ring of His-438 and the negatively charged phosphoryl oxygens may contribute to the observed shift in the onset of unfolding to a higher temperature compared to the native enzyme book make permanent up .
After amplification and discrimination, signals were directed to an ALV/5000E Multiple Tau Digital Correlator and time-intensity correlation functions recorded, typically for 600 s duration book make permanent up .
The protein bands were visualized through Coomassie R-250 staining book make permanent up .
Instead they have served just to keep the diffusive search within the smaller (d - 1) or (d - 2) dimensional space, once the first subdimensional search is over, then within a (d - 3) or (d - 4) dimensional space after the subsequent successful "adsorption" into the still-smaller subspace, and so on book make permanent up .
These intrinsic features of RNA folding reactions can be obscured due to the averaging inherent in conventional ensemble experiments book make permanent up .
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