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The purity of stock GR preparations was routinely verified by SDS-PAGE, followed by Coomassie-Blue staining
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The amide I' bands were assigned according to previous publications (24)
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Three-point calibration of lhe pH-meler was performed by using slandards from Radiometer
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As a consequence the obtained transition temperatures are strongly depending on the heating rates (for a more extended discussion on this subject see (17))
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DmAChE, for example, hydrolyses its natural substrate (turnover number ~1000 s^sup -1^) 50% faster than HuBChE ((31) and references therein)
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In the experimental situation, although the protein particle is expected to be monodisperse, in practice a limited aggregation is often observed
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This dependence of the binding of monocations is usually explained from the viewpoint of a decreasing hydrated radius of monocations in aqueous solutions (Ivanov et al
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Reference spectra of conformational limits
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Bis-ANS shows very strong fluorescence in response to all conformational states of TTR, with the strongest signal emanating from the rapidly formed monomeric A-state upon NaCl addition
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5M ammonium hydroxide (9:7:2, v/v) under nonreducing conditions
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This refolding may reflect partial reconstitution of the apoA-2:DMPC complexes
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Quality of striped bass, Morone saxatilis, eggs in relation to river source and female weight
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Plain x-ray revealed that an intact BB was lodged in her skull at the junction of the fight ethmoid sinus and septum (figure 1)
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. The precipitate was dissolved in a minimum amount of the butter and extensively dialyzed against the same buffer
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. As a result, for each sequence, we generated an ensemble consisting of 10^sup 4^ uncorrelated conformations
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. In contrast, CD spectroscopy revealed no differences for the unfolded states at the respective heating rates (Fig
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. Thus, pressure methods allow for the estimation in the volume associated with the Gα^sub s^(GDP) deactivation of the PLCβ-Gβγ complex even though the change in interaction energy between PLCb and Gβγ caused by Gα^sub s^(GDP) could not be detected by spectroscopic atmospheric pressure measurements
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. Untangling of amyloid architecture and intrafibrillar packing of the β-pleated sheets is the key issue in designing inhibitors of the fibrillar growth-pharmaceuticals that could prevent spreading of a misfolded protein in diseased tissues (Cohen and Kelly, 2003)
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.9 78EH-04/9865[degrees]20'Pseudopercis3448
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. Fine aluminum/silicon particles can become suspended in wet machining coolant
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. This broadening/tailing is evidence for occupation of two (or more) structures that are interconverting on a timescale similar to or slower than the average burst duration
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.5114,099Cumulative effect post- RP10,167
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