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. Details with regard to the intrinsic fluorescence experiments were the same as those described before (30)
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. At any of the explored pH, the percentages of β-hairpin (at 1693 cm^sup -1^) turns/loops/(0, π) β-sheet (at 1679 cm^sup -1^) and antiparallel β-sheet (at 1633 cm^sup -1^) remained essentially unaltered
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. No losses were detected for any sample
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. An alternative explanation is that in this concentration range, the presence of an interfacial layer of protein has a marked influence on the observed rheology
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. The results presented in this report describe the misfolding, oligomerization, and aggregation process of TTR that was induced from unfolded TTR through induction of the molten globule A-state
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. 33 K/min is sigmoidal and reaches a plateau at high temperatures, suggesting that the unfolding transition is complete at 99°C, yet the light-scattering curve recorded in the same experiment does not reach a plateau, and the signal continues to increase upon cooling from 99°C (Fig
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. Similar to the CD data, the light-scattering heating curves of the apoA-2:DMPC disks shift to lower temperatures at slower scan rates, indicating high activation energy for the disk fusion
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. Figure 4 shows a typical particle size distribution for these experiments
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. We conducted the subsequent tests for the effects of other factors on particle size using Isopar G, a dissolving temperature of 130°C, and a dissolving time of 2 hours
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. [FIGURE 1 OMITTED]Histological preparations were made from the ovaries of 175 females collected monthly from March 1996 through March 1997 to track seasonal ovarian development
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. , unequal elevations) which necessitated that the data be fitted with a parallel-line multiple linear regression model (Fig
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. Maturity stageMacroscopic descriptionHistological description1 ImmatureSmall and translucentOocyte cytoplasm intenselyovary, pink during monthsbasophilic
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. Moreover, the quasi-docked species observed in our study appears to form very rapidly, with a rate constant that is two to four orders of magnitude faster than the value reported for formation of the intermediate for the immobilized 4WJ ribozyme under similar conditions (Tan et al
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