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Column calibration was done with a set of eight proteins of known molecular weights (thyroglobulin, 669 kDa; ferritin; 440 kDa; alcohol dehydrogenase, 150 kDa; bovine serum albumin, 66 kDa; ovalbumin, 45 kDa; carbonic anhydrase, 29 kDa; trypsinogen, 24 kDa; and cytochrome C, 12 kDa) cologne french man perfume womens .
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