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Finally, the solution was dialyzed against TBS (Tris buffer (pH 7
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Until 2 M denaturant concentration
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Experimental data were corrected from small mismatches between the two cells by subtracting the buffer baseline before data analysis
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J Am Coll Cardiol 2006 (in press)(59) Asada K, Sasaki S, Suda T, et al
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A measure of conformational entropy change during thermal protein unfolding using neutron spectroscopy
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1, close to the isoelectric point, and pH 6
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We also tested the ability of Gα^sub s^ to remain membrane-bound under pressure
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Dipyrenylphosphatidylcholines as membrane fluidity probes
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Several works implicated importance of particular amino acid residues for insulin aggregation; for example, the bovine insulin, diverging from the human protein at three amino acid positions (A8 Ala^sup Bov^/Thr^sup Hum^, A10 Val^sup Bov^/ Ile^sup Hum^, and B30 Ala^sup Bov^/Thr^sup Hu^), aggregates more easily in vitro (Nielsen et al
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Amyloid libers are water-filled nanotubes
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Truncated staphylococcal nuclease is compact but disordered
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The pelvic fin was large and slightly shorter than the pectoral fin, whose margin was rounded
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In order to suppress non-specific binding of IgG, the sections were blocked with normal rabbit serum (Dako AJS, Denmark), diluted 1:5 in TBS for 20 minutes
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