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" As this article goes to press, more protest resignations, this time from the Carter Center's board of councilors, appear to be in the works
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. Fully unfolded monomers were obtained at higher concentrations of GdnHCl
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. However, it may also happen that at this denaturant concentration, some amount of protein has reached the completely denatured slate and the loss in the ellipticity value is due to the contribution from that fraction of the denatured protein
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. The reversibility of thermal transitions was tested by recording a new scan alter cooling the thermally denatured sample to 298 K, and comparing the thermal denaturation curve with that obtained in the first scan
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. The addition of the surfactant Tween 20 to the subphase abolished the shear elasticity as a consequence of the disruption of the adsorbed layer (60)
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. Some useful characteristics of DCVJ make this a very attractive complement and alternative to ThT for amyloid and aggregation studies
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. The FRET efficiency histograms described in this article were generated by using a two-channel data collection mode to simultaneously record donor and acceptor signals as a function of time, with a time resolution of 0
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- Keep on the Sunny Side collects 40 June Carter Cash performances dating from 1939 to 2003, from Carter Family through collaborations June did with Homer and Jethro, Cal Smith and, of course, Johnny Cash, plus solo work
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