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McPhillips; personal communication; March 2006; and V
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With respect to corresponding CD spectra we observed for both types of samples (i
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4 ms was used for (180 µm)^sup 3^ voxel spectra, and TE = 6
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John Wiley & Sons, Chichester
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Modifications induced upon oligomerization in high-frequency, small amplitude/localized modes, which do lie within the sensitivity of our experimental window, might be rather small
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Such work is ongoing in our laboratories, and data from misfolded TTR oligomers recorded from site-specific fluorescence labeling of cysteine mutants have provided size distributions with average rotation correlation times of ~ 150 ns (K
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Immediately after the incubation, the protein samples were diluted 400 times with ultrapure hydrochloric acid in water (pH 1
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The 70°C preparations yield populations of equally long, but 2
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© 2005 by the Biophysical Society0006-3495/05/02/1344/10 $2
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Each color reflected by the clouds -- pale hues of red, blue and green -- tells a separate story
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At 5 wt%, we observed that a continuous gel was not formed, as we had found with the higher polymer concentrations
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We produced one IPP and two EVA toners according to the optimized protocols discussed above
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Response variables = AF and RF,treatment factors = year, and covariate = age
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Because the two loop-carrying arms are in close proximity, it is likely that the quasi-docked conformation facilitates the formation of tertiary interactions between loops A and B
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The broad asymmetric peak shape observed for the SL 4WJ implies that extended undocked and quasi-docked states are in rapid conformational exchange, and our kinetic simulations indicate that the rate constant for formation of the quasi-docked state is in the range 10-41 × 10^sup 3^ s^sup -1^, whereas the corresponding reverse rate constant is in the range 3
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