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. The precipitate was collected this time by centrifugation at 8000 rpm for 45 min
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. At any of the explored pH, the percentages of β-hairpin (at 1693 cm^sup -1^) turns/loops/(0, π) β-sheet (at 1679 cm^sup -1^) and antiparallel β-sheet (at 1633 cm^sup -1^) remained essentially unaltered
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. GndHCl-induced unfolded states show for all α-amylases emission spectra with λ^sub max^ of ~348-349 nm which is nearly the value one would expect for fully accessible tryptophan, namely 350 nm
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. We refer to these plots in the following as Guinier plots, due to their analogy to the Guinier plots in SAS
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. Although less confined than CD4-F43, it is in close contact (In addition to the residues that are directly involved in intermolecular contacts or are in close proximity to the intermolecular interface, there are a few residues in gp120 that show large intraresidue entropy changes upon complexation (see Fig
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