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At first glance, these results are surprising since the highly denatured state is the starting point for in vitro folding reactions and yet no consideration of the denatured-state topology is required to account for the folding rates
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Powders were dissolved in buffer (for details see below) and all enzymes were purified by the use of a desalting column (PD-10, Sephadex G-25, Amersham Biosciences, Freiburg, Germany)
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With increasing concentration, the solution viscosity showed a progressive increase, a behavior interpreted as the approach to a colloidlike glass transition at ~60% w/w
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1 (10 mM buffer) and NaCl concentrations of 0 mM, 100 mM, and 1 M
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To enable conformational characterization of protein isolated from biological specimens such as samples from patients, noncovalent recognition using extrinsic dyes is necessary
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The stable intermolecular hydrogen-bond network (166 and 130 atoms for CD4 and gp120, respectively; for details see Table 1)
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, under conditions used for generating the FRET histograms in this article)
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