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If folding were strictly driven by the formation of local contacts (100-102)
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This feature is shared with Gaussian chains (39), although clear differences exist in the magnitude of and correlation between conformational fluctuations
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To perform studies in this direction the conformational stability of encapsulating structures at rather elevated temperatures (above 80°C) needs to be ensured
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Previous work by us and others have employed protein engineering and cysteine labeling to attach specific probes to proteins to record conformational changes (Hammarström et al
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2, B and C; Fig
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Moreover, the quasi-docked species observed in our study appears to form very rapidly, with a rate constant that is two to four orders of magnitude faster than the value reported for formation of the intermediate for the immobilized 4WJ ribozyme under similar conditions (Tan et al
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