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Under reducing conditions, the number of SH groups in urea-denatured yeast GR increases to six SH groups/monomer (Massey and Williams, 1965), indicating full exposure of all the Cys residues in the protein
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The thermal unfolding of ACYI might be a three-state process involving an aggregation-prone intermediate appearing at ~68°C
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The probable reason for the failure to deglycosylaic may be related to the fact that the terminal mannose residues are well rooted in the clefts and cavities on the protein surface, which made them inaccessible to the enzyme
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Can the differences quantified in Fig
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Scaling behavior and structure of denatured proteins
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In the vegetal cytoplasm, the aceturate peaks are still smaller than in the animal cytoplasm, concordant with the vegetal cytoplasm being even more stuffed with maternal macromolecules (31,39,40)
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After amplification and discrimination, signals were directed to an ALV/5000E Multiple Tau Digital Correlator and time-intensity correlation functions recorded, typically for 600 s duration
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4 µM for ANS, and 6
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Several amyloidogenic in vitro assays employ acidic pH for generation of amyloid fibrils; however, ThT shows very modest fluorescence at acidic pH when bound to protofibrillar amyloid of TTR (Fig
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The pleckstrin homology domain of phospholipase C-β^sub 2^ links the binding of Gβγ to the catalytic core
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By using model disks comprised of apoC-1 and DMPC, we showed that the heat-induced protein unfolding is a slow thermodynamically irreversible (or non-equilibrium) transition
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The hypergutter picture is also related to the topomer search model
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The low number of positive stations in spite of the intense sampling conducted within the area of distribution of P
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161ErrorEquality of elevation 0
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