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. This work was supported by grants from the Swedish Research Council (S
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. A total of 592 ichthyoplankton samples and 277 juvenile trawl samples were analyzed (Table 1)
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. After washing with PBS (100 [micro]L), biotinylated horse anti-mouse IgG (100 [micro]L) was added to the wells and incubated at room temperature for 20 minutes
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. Hence, the 4WJ ribozyme is unique in its ability to fold into a structure with arms A and B in close proximity, similar to the native ribozyme, even in the absence of specific loop-loop docking interactions
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. An additional step of affinity purification was done using a lactosyl-Biogel column to ensure the presence of only the active, refolded molecules in the rSBA sample finally obtained (21)
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. or 350 nm, after excitation at 280 and 295 nm, with a scan rate of 30 K/h
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. Fluorescence experiments at pH 7 and 9 showed a sigmoidal transition with T^sub m^ = 311 ± 4 K (Fig
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. The phosphatidylserine receptor from Hydra is a nuclear protein with potential Fe(II) dependent oxygenase activity
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. (15) recorded an MR spectrum from a (220 µm)^sup 3^ voxel within an A
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. In the vegetal cytoplasm, the aceturate peaks are still smaller than in the animal cytoplasm, concordant with the vegetal cytoplasm being even more stuffed with maternal macromolecules (31,39,40)
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. Hence, for any reasonable value of Δ^sup ex^ (up to 6 ppm), these residues are in fast exchange (k^sup -1^^sub ex^ [much greater than] Δω^sub I^)
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. For the high-resolution imaging, the AFM head (with optical block and base) was placed atop a commercially available active (piezo-actuated) vibrations-damping desk (from Halcyonics, Göttingen, Germany)
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