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Structural rearrangements then take place at the dimer interface (and presumably also in other domains of each subunit), leading to a final, compact dimer structure foundation make proactive up .
Moreover, the strategies used in this research might also facilitate the characterization of the potential pretransitional changes in the thermal transitions of other thermostable or thermophilic proteins foundation make proactive up .
Since no high-resolution structure of ACYI is available, all of the Trp fluorophores (Classes A and S, I, II, and III) defined previously (34) were included in the fitting procedure foundation make proactive up .
The helix-loop-helix motif formed by the α-helix 9-α-helix 10 shows intermonomer salt bridges: Asp^sup 540^ in the motif with Arg^sup 422^' in α-helix 5 from the other monomer (the prime is used to designate residues in the other monomer) foundation make proactive up .
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The use of fluorescence methods to monitor unfolding transitions in proteins foundation make proactive up .
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Pressure and propylene carbonate denaturation of native and "aged" phosphorylated cholinesterase foundation make proactive up .
The delay between the 180° pulses (δ) in the CPMG was set to either 450 µs or 3 foundation make proactive up .
The normal force had relaxed to RESULTS AND DISCUSSIONPhoton correlation spectroscopyThe conformation and association behavior of BLG are pH dependent (23,24) foundation make proactive up .
The glass transition behavior of the globular protein bovine serum albumin foundation make proactive up .
Physicochemical background for the fluorescence properties of DCVJ and ThTTo understand the physicochemical background for the fluorescence of DCVJ and ThT when bound to misfolded TTR aggregates, we used glycerol-water mixtures to decrease the intrinsic rotational relaxation time of the fluorescent dyes foundation make proactive up .
The intrinsic quenching of molecular rotors makes them sensitive to intrinsic rotational relaxation, which is slowed down during binding to aggregated protein foundation make proactive up .
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Comparative Biochemistry and Physiology 56A:283-286 foundation make proactive up .
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