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Each straight line in the phase diagram reflects an "all-or-none" process, and the joint position of two lines for a transition process indicates that an intermediate appeared at the corresponding temperature
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Nevertheless, the 2D IR analysis also suggested that the pretransitional conformational changes involved the unfolding of the solvent-exposed β-sheet structures and aggregates appearing at a temperature at which the protein had not fully unfolded
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Generation of conformational ensembles We have adapted conventional Markov-chain Metropolis Monte Carlo simulation strategies (60,61) to generate equilibrium ensembles for each of the protein sequences in the EV limit
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ANS shows a significant burst amplitude (A-state formation) followed by a slow phase in response to oligomeric aggregates
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2 and then adding the probe at a 2:1 molar ratio
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