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The N-terminal domain showed basically the same structure as the EIN^sup ec^ (8,9), since the alignments for homology modeling showed only minor deletions in loops between α-helix I and α-helix 2, α-helix 2 and α-helix 3, and α-helix 3 and α-helix 4 (Fig
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In conclusion, from examining azurin mutants of lower thermodynamic stability and greater internal flexibility than the WT, it was found that the perturbation of the native fold in the frozen state, evidenced by the capacity to bind ANS, is enhanced by the plasticity of the globular structure and correlates with the lability to freeze-thawing
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The crystal structures of Ca^sup 2+^/CaM show an extended dumbbell shaped molecule, in which its two globular domains are connected with a long α-helix (1)
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The ability of this model to fit the data indicates that the proteinprotein interactions under these conditions are predominantly repulsive
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But, as we saw in the last section, some residues may provide dimensional reduction that significantly affects the overall rate via their nonnative interactions, without affecting the transition state itself
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