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Since gSBA and rSBA both exist as tetramers with identical ligand binding specificities and macromolecular properties, the change of the mode of unfolding from a two-state process to a three-state process and the decrease of T^sub m^ by 26°C for the thermal unfolding of the protein of the kilter implicate the covalenlly linked glycans for the stability of gSBA girl putting on make up .
In EI^sup sc^, a single peak was observed in CD and DSC experiments, but in the pH range where the thermal transitions could be followed (pH 7-9), two different peaks were not observed; further, the T^sub m^ of the sole transition observed was pH-indcpendent between pH 7 and 9 (Fig girl putting on make up .
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Thus, removing an interaction is indeed expected to have only a small effect on the affinity girl putting on make up .
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