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But why lose them? What's more, since so many species of cave-dwelling fish, not at all closely related, have lost their eyes, the phenomenon seems to be neither an accident devolution nor an isolated event
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Interestingly, considerable conformational changes were observed before the enzyme was fully inactivated by high temperature
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As a consequence, several special characteristics of conformational ensembles for polymers in the EV limit-and, by extension, of highly denatured proteins-are not anticipated by Flory's theory
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6, which shows that deviations from rodlike behavior occur for all sequence separations greater than a single residue
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To derive thermodynamic parameter from spectroscopic data it is crucial whether the signal changes during the unfolding process are proportional to the molar fractions of the folded and unfolded protein
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In contrast all thermal unfolded states (with the exception of those obtained from PPA) exhibit λ^sub max^ values around 340 nm, which correspond to much smaller red shifts upon unfolding
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This result is shown for BAA in Fig
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Although considerable evidence suggests that only partial destabilization of insulin is the prerequisite for its aggregation (Ahmad et al
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Such a predisposition may not be enough to cause oxidation during mix storage, when exposure to oxygen is minimal
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Lastly, kinetic information about equilibrium interconversion of subpopulations (on microsecond to millisecond timescales) is uniquely available from spFRET data
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Figure 1 illustrates total DNA (Fig
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