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- Thirty-one years later she confessed how terrified she was: "San Quentin is a maximum-security prison
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. The underlying assumption is that the highly denatured-state ensemble for two-state proteins can be mimicked using our EV model
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. Since the folding process is complex in multidomain proteins, where each domain may be able to refold independently and interdomain interactions may affect the overall folding process, EI can be used, in addition, as a model protein to improve our knowledge of protein folding and assembly of multidomain-oligomeric proteins
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. Samples were frozen in liquid nitrogen and stored at -80°C
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.3 M^sup -1^ and v = 0
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. All α-amylase structures bear multiple tryptophan residues more or less equally distributed over the whole structure (see Table 1 and Fig
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