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Deuterated samples were from Cambridge Isotope Laboratories (Andover, MA)
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No significant difference was observed when the scanning rate was increased from 0
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, glycans attached to subunit X forming hydrogen bonds with residues of subunit X), whereas the rest are intersubunit interactions
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Thermodynamics and kinetics of non-native interactions in protein folding: a single point mutant significantly stabilizes the N-terminal domain of L9 by modulating non-native interactions in the denatured state
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J Cell Biol 2001; 155:649-660(22) Devitt A, Parker KG, Ogden CA, et al
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Macromolecular binding, however, prevents an exact quantification of the drug concentration-especially at or near the lower detection limit-in the different cellular compartments, which would be required for a detailed kinetic analysis of drug uptake
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Dissecting the vibrational entropy change on protein/ligand binding: burial of a water molecule in bovine pancreatic trypsin inhibitor
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At pHs in the vicinity of the isoelectric point (pH 5
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MethodsSamples for fluorescent microscopy were illuminated with 365 nm ultraviolet light, and fluorescence images of DNA molecules were observed using a Zeiss Axiovert 135 TV microscope equipped with a 100× oil-immersed lens and recorded on S-VHS videotape through a Hamamatsu SIT TV camera
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It should however be noted that Bis-ANS still fluoresces very strongly in the presence of large aggregates (Figs
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The negative slope in the heating and cooling curves in Fig
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2[degrees]C [[+ or -] 2
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The process was similar to our process, but a different solvent was used
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The low pigment concentration in the toner may be associated with the removal of large particles by sieving, which may have contained a larger proportion of the pigment
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(1-8) Most victims (64%) are taken to an emergency department in a private vehicle; as many as 8% are transported by emergency medical services
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For histological and immunohistochemical analyses samples were fixed in 4% (w/v) paraformaldehyde in 0
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