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Nevertheless, the 2D IR analysis also suggested that the pretransitional conformational changes involved the unfolding of the solvent-exposed β-sheet structures and aggregates appearing at a temperature at which the protein had not fully unfolded
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This investigation was supported by Grant 30500084 from the National Natural Science Foundation of China, and Grant 101023 from the Fok Ying Tong Education Foundation
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ANS Fluorescence Detects Widespread Perturbations of Protein Tertiary Structure in IceGabellieri, EdiABSTRACT Freeze-induced perturbations of the protein native fold are poorly understood owing to the difficulty of monitoring their structure in ice
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Indeed, several lines of evidence indicate that both pressure-induced and thermal denaturation of HuBChE are multistep processes that are initiated by penetration of water into the active-site gorge, leading to protein swelling and, eventually, unfolding (18,53,54)
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The measured decays were lit to exponential decays of two or three components depending on the complexity of the decay trace
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Each has advantages and drawbacks, but it is not clear whether each applies to a restricted subset of real cases, or whether all might have something to say about the folding of any one protein
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We note here that although we have interpreted peak shifts and broadening in terms of distance changes and ribozyme subpopulations, respectively, peak shifts could also potentially be caused by solvent effects on dye photophysics, and corresponding changes in the correction factor [gamma] (see Materials and Methods), as well as peak broadening by effects of [kappa]^sup 2^, the orientation factor (Deniz et al
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