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Hence, the first transition is related to the dissociation of the tetrameric unit to constituent monomers, whereas the second one represents opening up of the individual subunits
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Folding and association of oligomeric and multimeric proteins
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The diminazene peaks (b and c) appear at 6
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12 ml g^sup -1^
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1, close to the isoelectric point, the osmotic pressure was significantly lower at lower ionic strengths (Fig
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The glass transition behavior of the globular protein bovine serum albumin
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We have found that for membranes composed of lipids with electrically neutral or negatively charged head groups, no change in surface charge could be detected at neutral pH from 1 to 2000 bar
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Studies using mutants are underway to better identify the residues responsible for these interaction volumes
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1, A and B), confirm that cold denaturation is an intrinsic property of the marginally stable S-S linked apoA-2 dimer
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This work was supported by National Institutes of Health grant No
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Other chemicals of suprapur or ultrapure grade were obtained from VWR (Merck GmbH, Darmstadt, Germany)
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Given the height scaling used in these measurements, the elongated species are not loosely interacting groups of single particles, but, instead, unified entities
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