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. Besides, complex formation between GR and NADPH was only detected in the dimeric form of the enzyme, which would argue that NADPH does not participate directly in the process of GR folding/assembly
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. The enzymatic activity was determined by measuring the absorbance at 238 nm accompanied with hydrolysis of the substrate and using the molar absorption coefficient ε^sub 238^ = 185 M^sup -1^cm^sup -1^ as reported by Kordel and Schneider (5,6), except that chloroacetyl-L-leucine was used instead of pure-L-enantiomorph
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. For the longest sequence in our data set-the sequence of villin-for which N = 126, generation of the desired conformational ensemble takes ~ 20 h on a single 2
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. (20), R^sub 2^ is measured for two settings of δ (we used 2δ^sub 1^ = 450 µs and 2δ^sub 2^ = 3
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. Pressure has profound effects on the highly compressible membrane interior promoting phase transition to gel phases and, for the studies presented here, the phase transition will occur ~1
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. ResultsThe dependent variables were analyzed using two way mixed-model analyses of variance (ANOVA, Advice/No-Advice x Block) unless otherwise indicated
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