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. For each of the sequences shown in Table 1, we computed an ensemble-averaged Kratky profile
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. Measuring unfolding of proteins in the presence of denaturant using fluorescence correlation spectroscopy
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. J Clin invest 1995; 96:1631-1638(47) Schrijvers DM, De Moyer CR, Kockx MM, et al
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. Molecular confinement influences protein structure and enhances thermal protein stability
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. Experiments conducted with copper-free azurin from Pseudomonas aeruginosa and mutants I7S, F110S, and C3A/C26A correlate the magnitude of the ice-induced perturbation, as inferred from the extent of ANS binding, to the plasticity of the globular fold, increasing with less stable globular folds as well as when the flexibility of the macromolecule is enhanced
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. Ice-induced ANS binding to other proteins To examine whether the ice-induced perturbation observed for azurin is common to proteins in general, ANS binding experiments were extended to a set of seven other proteins including monomeric BSA, α-amylase and β-gal, dimeric LADH and tetrameric ALD, YADH, and LDH
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. All frogs used were healthy, and all experimental procedures involving animals were performed in accordance with the institutional ethical guidelines
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. The pleckstrin homology domain of phospholipase C-β^sub 2^ links the binding of Gβγ to the catalytic core
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. The loss of these specific tertiary docking interactions makes these SL constructs mimics for the undocked state of the corresponding WT ribozymes
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. Int J Dev Biol 1999:43:59-67
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