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. The ANS binding test extended to other proteins confirmed a widespread stressed condition of the globular structure in ice, indicating that oligomers' proteins are more susceptible to unfolding than monomers', presumably because the macromolecule can be further destabilized by subunit dissociation
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. The FRET efficiency histogram of the GnI variant also exhibits two distinct subpopulations, with mean E values that are similar to the wild-type, but the amplitude of the high FRET peak is substantially reduced relative to the low FRET peak (Fig
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. Given the equivalence between the EV limit and highly denatured states, our ability to simulate conformational ensembles in the EV limit, with full atomic detail, has direct bearing on the development of an accurate physical picture of conformations accessible to denatured proteins
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. We also determined the pressure range in which the proteins remain membrane bound: PLCβ binding was stable throughout the 1-2000 bars range, Gβγ binding was stable only at high membrane concentrations, whereas Gagr;^sub s^(GDP) dissociated from membranes above 1 kbar
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