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A distinct shoulder ([lambda]^sub max^ ~ 305 nm) corresponding to tyrosine emission (18 Tyr residues in each GR subunit) appeared in the spectrum mineral mine make up .
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Large unilamellar vesicles (LUVs) were freshly prepared by extrusion through a 100-nm pore filter before every experiment mineral mine make up .
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Thus we chose a dissolving temperature of 80°C mineral mine make up .
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