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. the intensity of the fluorescence spectra decreased (Fig
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. J Exp Med 2000; 191:515-528(18) Okazawa H, Motegi S, Ohyama N, et al
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. As already mentioned above, the unfolding transition of proteins is not only characterized by the disappearance of the well-defined three-dimensional structure but can also cause distinct changes in the compactness of the unfolded structures or may induce pronounced protein aggregation
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. Thus, activation by an agonist through the PLCβ-G-proteins pathway affects the activity of a host of Ca^sup 2+^-sensitive enzymes
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