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5 C to avoid the possible artifacts caused by large band shifts accompanied with slight intensity changes in this temperature range (see also Fig starting a barber shop .
The dialyzed solution was centrifuged at 8000 rpm for 15 min and the clear supernatant was loaded on a lactosylamine Biogel P-150 column preequilibrated wilh buffer (21) starting a barber shop .
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That a two-contribution model is needed follows from a comparison of average experimental and theoretical R^sub 1^ values starting a barber shop .
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INTRODUCTIONMany diseases are caused by protein misfolding starting a barber shop .
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