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. The fluorescence emission of GR undergoes a marked red-shift between 1 and 3 M GdnHCl (with a transition midpoint at ~2
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. The ANS and DCVJ fluorescence thereafter increase over time as oligomers and protofibrils form and reach a plateau after a few hours
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. Slowing down the scan rate also leads to a reduction in the CD amplitude in the cooling curves below 80°C (Fig
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