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When his agent asked if more guards were needed to protect the stage, the security chief replied that one hundred, even two hundred guards couldn't control a thousand, spring-loaded prisoners if things spun out of control
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. * Sykes: There are myriad reports that are utilized to effectively manage the revenue cycle
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. Of course, NOAA scientists can't count all the fish in the sea, so they do a lot of sampling and computer modeling
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. 5 M GdnHCl and partially folded monomers at 3 M GdnHCl
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. From the difference contact maps, we find that upon folding, specific nonnative local contacts have to be broken (weakened) to make native, nonlocal spatial contacts
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. Azurins stocks were dialyzed and stored in Tris HCl, 10 mM, pH 7
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. Simulations of protein folding and unfolding
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. For instance, the barnase (45) and oxalocrotonate tautomerase (46) bind specifically to their rigid target to form a specific complex with essentially one overall conformation
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. Jamming phase diagram for attractive particles
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. 0 but only when the pH is increased to pH 7
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. Similar to the results obtained for the proteins labeled with other probes, the titration curves for the proteins labeled with CPM showed the appropriate shift in midpoint when the initial concentration of CPM-labeled protein was changed, thus showing that the changes in coumarin fluorescence reflect protein-protein associations
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. Gα^sub s^ is modified by two palmitoyl chains (Kleuss and Krause, 2003) which are thought to contribute to its membrane stability
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. In principle, the protein-protein interface would be the same whether the protein is freely diffusing in solution or confined on a membrane surface, and thus we expect that for a specific association the volume change would be similar if the proteins were freely diffusing or confined to a membrane surface
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. Address reprint requests to Roland Winter, Tel
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. brasilianus was found by itself only at three stations
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. The point is continually made that the goal of function and improvement must always be given priority over that of enhancing beauty
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. And health and beauty is growing at 61 percent year over year
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. Type 2 RP was applied to the NSAIDs in November 1995
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. SUPPLEMENTARY MATERIALThe following supplementary material is available for this article online: TABLE S1
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. Held in the nation's capital, the annual gathering reflected its policy-making backdrop in both mood and content
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. The researchers also found that work activities, including publication frequency, teaching experience and professional service, were not necessarily impacted by gender roles
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. Arizona, for example, forbids anyone younger than 21 to gamble in any form
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. When you get into personal morality, the person gambling is already getting beat
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. Covalently hound sugars in proteins range from small monosaccharides, especially on nuclear and cyloplasmic proteins, to substantially complex molecules on extracellular N- or O-linked glycoproteins, including many lectins
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. The protein concentration used was 0
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. This behavior is expected to hold for all |i - j| > n^sub s^, where n^sub s^ denotes the number of residues over which sequence context is important
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. The excitation light, provided by a Cermax xenon lamp (LX150UV, ILC Technology, Sunnyvale, CA), is selected (λ^sub ex^ = 350 nm for ANS and 285 nm for azurin fluorescence, respectively, 6 nm bandwidth) by a 0
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. We conclude that stabilization, and corresponding effects on the MSDs of HuBChE, by solvent/buffer and by the covalently bound inhibitor involve two different mechanisms
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. Solvent stabilization by salting-out ions is a consequence of a decrease in free energy of the native state, due to both entropic and enthalpic contributions; in particular, the effect on hydration water is expected to be mainly entropic, whereas the observed decrease in protein MSDs (44) indicates a tighter packing of the apolar residues in the core of the protein as suggested for hydrophobic stabilization by calorimetry experiments (59)
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.1 (10 mM buffer) and NaCl concentrations of 0 mM, 100 mM, and 1 M
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. Both types of G-protein subunits were destabilized under pressure, suggesting an inefficient packing with the membrane surface
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. Resolution of a signal transfer region from a general binding domain in Gβ for stimulation of phospholipase C-β2
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.Homogenization should produce a fat globule size distribution of 1-2 microns
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. A temperature probe measures the incoming part temperature
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. Neophytes like me imagine that the best players possess the qualities we saw or wanted to see in our father figures: mental toughness, boldness, steadfastness
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. Alternatively, poor play could be a function of fatigue
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. Kushner portrays the Magic contingent's gambling success as a revenge-of-the-nerds-type triumph (the book's subtitle is How a Gang of Geeks Beat The Odds and Stormed Las Vegas), and valiantly tries to invest this success with more importance than I think it actually deserves
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. In the present study, players also tended to raise their bet following a win and to decrease their bet after a loss
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